Next Generation Sequencing

What is Next Generation Sequencing?
Next Generation Sequencing (NGS) is a technology that can sequence millions of pieces of DNA in parallel in rapid fashion. Key aspects of NGS is that it has ...
Sat, 3 Aug, 2019 at 3:20 PM
What is an amplicon?
A short segment of DNA duplicated over and over, i.e. amplified, via PCR. In metabarcoding, amplicons are the segments of DNA of interest that we sequence.
Sat, 3 Aug, 2019 at 3:20 PM
What is an OTU?
OTU stands for “Operational Taxonomic Unit”. OTUs are clusters of sequences that are relatively similar and may represent multiple species. Since a single O...
Sat, 3 Aug, 2019 at 3:21 PM
What is a primer?
A primer is a short section of DNA that has been synthesized. Its purpose is to bind to DNA segments during PCR and initiate amplification. Primers are used...
Sat, 3 Aug, 2019 at 3:22 PM
What is BLAST?
BLAST is a technique that can be used to match a particular DNA sequence with sequences in a reference library. BLASTing allows one to figure out what speci...
Sat, 3 Aug, 2019 at 3:22 PM
What is metabarcoding?
Metabarcoding is a technique to quantify the abundance of different species in mixtures. It uses PCR and primers that amplify a particular short region DNA ...
Sat, 3 Aug, 2019 at 3:24 PM
What is an ESV?
An ESV is an Exact Sequence Variant. The sequence represented by an ESV is generated from a denoising algorithm. Whereas OTUs are clusters of similar sequen...
Sat, 3 Aug, 2019 at 3:36 PM
Which Illumina machines do you use?
We use the iSeq, MiSeq, and NovaSeq.
Wed, 23 Sep, 2020 at 3:56 PM
Which primer sets do you typically use?
NGS:  16S-bacteria trnL-plants, pollen 23S-phytoplankton ArthCOI-insects, macroinvertebrates Ac12S-vertebrates uniCOI-zooplankton 18S-parasites ITS-fungi M...
Wed, 23 Sep, 2020 at 11:23 AM
Can I submit a primer set for you to use?
You can, but we will need to run it by our bioinformatics team first to be sure it is compatible. Please contact us with this kind of situation. 
Thu, 24 Sep, 2020 at 3:57 PM