Next Generation Sequencing

What is Next Generation Sequencing?
Next Generation Sequencing (NGS) is a technology that can sequence millions of pieces of DNA in parallel in rapid fashion. Key aspects of NGS is that it has ...
Sat, 3 Aug, 2019 at 3:20 PM
What is an amplicon?
A short segment of DNA duplicated over and over, i.e. amplified, via PCR. In metabarcoding, amplicons are the segments of DNA of interest that we sequence.
Sat, 3 Aug, 2019 at 3:20 PM
What is an OTU?
OTU stands for “Operational Taxonomic Unit”. OTUs are clusters of sequences that are relatively similar and may represent multiple species. Since a single O...
Sat, 3 Aug, 2019 at 3:21 PM
What is a primer?
A primer is a short section of DNA that has been synthesized. Its purpose is to bind to DNA segments during PCR and initiate amplification. Primers are used...
Sat, 3 Aug, 2019 at 3:22 PM
What is BLAST?
BLAST is a technique that can be used to match a particular DNA sequence with sequences in a reference library. BLASTing allows one to figure out what speci...
Sat, 3 Aug, 2019 at 3:22 PM
What is metabarcoding?
Metabarcoding is a technique to quantify the abundance of different species in mixtures. It uses PCR and primers that amplify a particular short region DNA ...
Sat, 3 Aug, 2019 at 3:24 PM
What is an ESV?
An ESV is an Exact Sequence Variant. The sequence represented by an ESV is generated from a denoising algorithm. Whereas OTUs are clusters of similar sequen...
Sat, 3 Aug, 2019 at 3:36 PM