Jonah Ventures

FAQ 6

• Can you do genotypic eDNA for populations and sub-populations?
• What metrics are available for an Index of Biotic Integrity (IBI)?
• What types of substrate can you process for eDNA?
• Are there educational materials that I can use to teach eDNA in my classroom?
• How long after you receive a sample can I expect results?

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Getting Started 2

• How do I fill out the Sample Submission sheet?
• Can I write on the sampling container?

General 1

• What is eDNA?

Next Generation Sequencing 13

• What is Next Generation Sequencing?
• What is an amplicon?
• What is an OTU?
• What is a primer?
• What is BLAST?

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Quantitative PCR 10

• What is quantitative PCR or qPCR?
• Why do you use Real-Time PCR instead of Droplet Digital PCR?
• Why do you use Real-time qPCR vs end point PCR?
• What do you do for quality controls?
• What qPCR assays do you offer?

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Isothermal 1

• What isothermal assays do you have?

General 4

• Which species have you done diet analysis for?
• Can you find out more than just diet from a fecal sample?
• Can you determine what lichens my animal is eating?
• How do you look at what is in a honey sample?

Sampling 15

• What is the best way to sample fecal material?
• Do I need to know exactly how many samples I will be submitting ahead of time?
• Can I send you samples in other containers such as plastic bags?
• What if I am sending swabs and they don’t fit?
• Should I write more information on the vial?

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Lab 5

• What primer pairs do you use?
• Should I run replicates?
• Should I keep duplicates?
• How many PCR replicates do you normally run?
• Do you archive samples?

Analysis 0

Lab 2

• What primer pairs do you use?
• Where do I ship samples?

General 4

• How long does DNA stay in the water?
• How far downstream from an organism can we detect the DNA?
• What fish species do you have in your library?
• There are over 1300 fish species in the United States. Your library only has about 500. What are you doing to fix the discrepancy?

Sampling 16

• What do you include when you send a box of supplies for sampling?
• What is the sampling protocol for eDNA?
• Are there any aids in pushing water through the syringe? My hand is starting to hurt.
• Can we use filters with different pore sizes?
• How should samples be stored?

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Lab 5

• What species can you detect with qPCR?
• What primer pairs do you use for NGS?
• Where and how do I ship my samples?
• Do you archive samples?
• What taxa can you detect with NGS?

Analysis 3

• Can you calculate IBIs with eDNA data?
• Why didn't you find all of the species at my site?
• Why did you find something that isn't at my site?

General 5

• What assays do you have for Source-Tracking?
• How do your methods compare to products that detect Enterococcus, E. coli, and fecal coliforms?
• Is there a difference between what Jonah Ventures can find out and the fecal coliform tests?
• Is this method certified by the EPA?
• Are regionally specific libraries used to analyze fecal pollution sources?

Analysis 3

• Can you separate the different strands of E. coli?
• What are the taxa combinations Jonah Ventures can do?
• What types of results can I expect?

General 8

• Help, I can't log in to my account!
• I haven't received an email about my account. Is something wrong?
• Where can I access the data portal?
• Will I receive a notification when my sample is received?
• Can I get a replacement if I lost a kit component?

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JonahWater 6

• What is in the eDNA sampling kits?
• What is included in the educational materials for the JonahWater kit?
• Do the kits have a shelf life?
• At what temperature should the kits be kept at before use?
• Are the kits susceptible to high/low temps?

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JonahAnimal 2

• What is in your JonahAnimal kit?
• What is included in the educational materials for the JonahAnimal kit?

General 13

• How do I read my NGS report?
• What is an ESV?
• Why are you switching from reporting OTUs to ESVs?
• Are there any drawbacks to ESVs?
• Should I consider every ESV to be real?

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Interpreting Data 1

• How to interpret NGS data

General 1

• How do I read my qPCR report file?

Consulting 1

• Can you help me with my eDNA project?

Legal 2

• Would you be willing to sample as a neutral party?
• Are your scientists non-biased?

qPCR 2

• qPCR report explained
• List of qPCR assays

NGS 2

• NGS report explained
• NGS Assays

Sampling Instructions 3

• Water Sampling Instructions
• Honey sampling instructions
• Fecal sampling instructions

Classroom 3

• Diet classroom activity and lesson plan
• Aquatic eDNA lesson plan and activity (fish)
• Educational fliers, fish and diet

Aquatic Documents 4

• Macroinvertebrate traditional vs. eDNA methods
• Aquatic eDNA method selection guide.
• Source-tracking for waterkeepers flyer
• NGS fish sequence library