Jonah Ventures

General 4

• How long does DNA stay in the water?
• How far downstream from an organism can we detect the DNA?
• What fish species do you have in your library?
• There are over 1300 fish species in the United States. Your library only has about 500. What are you doing to fix the discrepancy?

Sampling 16

• What do you include when you send a box of supplies for sampling?
• What is the sampling protocol for eDNA?
• Are there any aids in pushing water through the syringe? My hand is starting to hurt.
• Can we use filters with different pore sizes?
• How should samples be stored?

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Lab 5

• What species can you detect with qPCR?
• What primer pairs do you use for NGS?
• Where and how do I ship my samples?
• Do you archive samples?
• What taxa can you detect with NGS?

Analysis 3

• Can you calculate IBIs with eDNA data?
• Why didn't you find all of the species at my site?
• Why did you find something that isn't at my site?