Sampling

What is the sampling protocol for eDNA?
At your site, sample 60 mL of water with the syringe. Attach the syringe filter and push water through. Repeat until you cannot push any more water through....
Mon, 5 Aug, 2019 at 3:57 PM
Are there any aids in pushing water through the syringe? My hand is starting to hurt.
You don’t have to push too hard in the end. The last few mL don’t help that much. But, when you have a lot of samples to push through in a day, sometimes yo...
Mon, 5 Aug, 2019 at 3:58 PM
Can we use filters with different pore sizes?
Our typical kit includes a filter with 1µm pores. For some specialized questions, filters with different pore sizes are helpful. Sometimes for pico plankton...
Mon, 5 Aug, 2019 at 3:59 PM
How should samples be stored?
Desiccation at room temperature is sufficient to stabilize DNA. Samples can additionally be frozen if desired before sending them in to be analyzed.
Mon, 5 Aug, 2019 at 3:59 PM
Do we need different kits for NGS and qPCR?
Nope.
Mon, 5 Aug, 2019 at 3:59 PM
When can I stop pushing water?
We typically stop pushing water when our hands start to hurt or we get to about 1 drop per second being pushed through. The amount of extra water pushed thr...
Fri, 9 Aug, 2019 at 2:35 PM
Is it better to use the bar clamp than my hand to push water through the filter?
Our testing shows that you can push about 10% more water if you use the bar clamp than our hands. Even if we assume that all that extra pushing at higher pr...
Fri, 9 Aug, 2019 at 2:39 PM