Why do you use Real-time qPCR vs end point PCR? : Jonah Ventures

Why do you use Real-time qPCR vs end point PCR? Print

Created by: Nicole Andrea Witzel

Modified on: Wed, 23 Sep, 2020 at 2:59 PM

In Real-time qPCR, we run the reaction for 50 cycles and monitor its progress during each cycle to examine the efficiency of our assays. Because we can monitor the progress during each cycle, we can make an estimation the number of copies in the original sample.

In end-point PCR, the reaction is run for as many cycles as possible and then checked at the very end for amplifications. End-point PCR allows you to see positives, but we do not get the concentration of DNA.

Nicole is the author of this solution article.

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Why do you use Real-time qPCR vs end point PCR? Print

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