Quantitative PCR

What is quantitative PCR or qPCR?
qPCR is similar to regular PCR, but with each cycle the qPCR machine quantifies the amount of fluorescence that is generated by a probe that attaches to a s...
Sat, 3 Aug, 2019 at 3:23 PM
Why do you use Real-Time PCR instead of Droplet Digital PCR?
In short, after extensive research, we found no significant difference in the results between Real-Time PCR and ddPCR that would justify us spending signifi...
Wed, 16 Dec, 2020 at 10:18 AM
Why do you use Real-time qPCR vs end point PCR?
In Real-time qPCR, we run the reaction for 50 cycles and monitor its progress during each cycle to examine the efficiency of our assays. Because we can moni...
Wed, 23 Sep, 2020 at 2:59 PM
What do you do for quality controls?
We run a comprehensive standard curve with each plate that we run to make sure that each run is working correctly.  
Wed, 16 Dec, 2020 at 11:21 AM
What qPCR assays do you offer?
Here is a list of all of the assays we offer at this time though we are always adding more!  If you are interested in having us run a different assay, pleas...
Wed, 23 Sep, 2020 at 2:13 PM
What testing or validation do you do for qPCR?
To create a qPCR assay, we first turn to the literature and see if anyone else has made an assay. If not, our bioinformatician will create one for testing. ...
Wed, 16 Dec, 2020 at 11:18 AM
Do you use positive controls?
We use positive controls when developing our assays and run standard curves with each and every qPCR run.
Wed, 16 Dec, 2020 at 10:15 AM
You don't offer a qPCR assay for my species. Can you create one for me?
Yes, we do offer this service at very reasonable prices. We keep these costs low for clients to support future research and support. Please contact us for m...
Wed, 23 Sep, 2020 at 3:54 PM
What types of files can I expect and how do I interpret the results?
For qPCR, we send you a full report detailing the amount of copies of target DNA found in each sample as well as the calibration curves that were run alongs...
Mon, 24 May, 2021 at 3:41 PM
Can you sequence for specific pathogens or antibiotic resistant genes?
We do not offer these services at this time, but we would be happy to point you toward a company who does. 
Wed, 23 Sep, 2020 at 3:53 PM