What primer pairs do you use?
For diet, we typically use trnL primers for plants, ITS primers for fungi, COI primers for insects, and 12S primers for vertebrates. For parasites, we typi...
Sat, 3 Aug, 2019 at 3:32 PM
Should I run replicates?
Scientifically, our general rule of thumb is to maximize statistical power. That means run as many independent samples as possible. Once the number of indep...
Mon, 5 Aug, 2019 at 3:54 PM
Should I keep duplicates?
We will archive samples until they are successfully sequenced. Samples are minimally handled and our techniques minimize the likelihood loss or contaminatio...
Mon, 5 Aug, 2019 at 3:54 PM
How many PCR replicates do you normally run?
Most animal samples are run with one PCR replicate. We can run samples with triplicate PCR pooled or unpooled. Contact us for pricing.
Mon, 5 Aug, 2019 at 3:55 PM
Do you archive samples?
We try to keep samples as long as we can to retest samples if need be, but when our freezer gets full, we need to throw out old samples. If you specifically...
Tue, 15 Dec, 2020 at 2:36 PM