General
An ESV is an Exact Sequence Variant. The sequence represented by an ESV is generated from a denoising algorithm. Whereas OTUs are clusters of similar seque...
Mon, 5 Aug, 2019 at 4:01 PM
We found that using ESVs reduces the number of items in results by 60%, e.g. 60% of the OTUs are a result of noise as opposed to likely being real. That mea...
Mon, 5 Aug, 2019 at 4:02 PM
The only thing we have seen so far is that some sequences that end in a long repeat of A (poly-A) are being separated as unique. We do not know if this is a...
Mon, 5 Aug, 2019 at 4:02 PM
We are agnostic on whether every sequence is real or not, but many low-abundance ESVs can still be excluded with justification. For example, if you are exam...
Mon, 5 Aug, 2019 at 4:02 PM
The original ESV paper from Robert Edgar: https://www.biorxiv.org/content/10.1101/081257v1 Paper comparing ESVs and OTUs: https://peerj.com/articles/5364/ ...
Mon, 5 Aug, 2019 at 4:03 PM
Sometimes, the species actually is there. Often it isn’t, but more than likely some species closely related to species in the listed genus is found at your ...
Mon, 5 Aug, 2019 at 4:03 PM
Some samples just do not amplify well – either do an insufficient amount of plant DNA in the sample or because it was difficult for us to get sufficiently c...
Mon, 5 Aug, 2019 at 4:03 PM
Yes, but it is less likely to be a species in that genus. The OTU is based on a consensus sequence that represents a most probable sequence and a number of ...
Mon, 5 Aug, 2019 at 4:04 PM
If there is no match in the library, this means that no species that have been sampled match the consensus sequences at some level of certainty. Generally, ...
Mon, 5 Aug, 2019 at 4:04 PM