Can you resolve species within a genus with NGS?
It depends on how different the sequences of the species are. Sometimes. Even if the reference file only matches to one species, that might be because there...
Mon, 5 Aug, 2019 at 4:05 PM
How do I manually BLAST a sequence?
The easiest is to go to: Enter in the sequence for an OTU. Results should follow.
Mon, 5 Aug, 2019 at 4:05 PM
Is there any significance to one sample having more total reads than another?
Not really. As long as there is more than ~500 or 1000 reads, the total number of reads conveys little information. Only relative abundance should be compar...
Mon, 5 Aug, 2019 at 4:05 PM