If there is no match in the library, this means that no species that have been sampled match the consensus sequences at some level of certainty. Generally, this is reported as an “unknown” in a paper, but more work can be done to narrow down what species they might represent. First, we’ll supply the sequence to you. You can go to the NCBI website and look to see what species have the closest match and try to interpret that in the context of your samples. Or, you can collect leaves/individuals of species that are likely to match that unknown. We’ll sequence these and rerun the bioinformatics to see if the unknowns now match.
What if there is no match between an OTU or ESV ID and anything in the library? Print
Created by: Joseph Craine
Modified on: Mon, 5 Aug, 2019 at 4:04 PM
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