Jonah Ventures
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Knowledge base
General
FAQ
6
Can you do genotypic eDNA for populations and sub-populations?
What metrics are available for an Index of Biotic Integrity (IBI)?
What types of substrate can you process for eDNA?
Are there educational materials that I can use to teach eDNA in my classroom?
How long after you receive a sample can I expect results?
View all 6
Getting Started
2
How do I fill out the Sample Submission sheet?
Can I write on the sampling container?
DNA 101
General
1
What is eDNA?
Next Generation Sequencing
13
What is Next Generation Sequencing?
What is an amplicon?
What is an OTU?
What is a primer?
What is BLAST?
View all 13
Quantitative PCR
10
What is quantitative PCR or qPCR?
Why do you use Real-Time PCR instead of Droplet Digital PCR?
Why do you use Real-time qPCR vs end point PCR?
What do you do for quality controls?
What qPCR assays do you offer?
View all 10
Isothermal
1
What isothermal assays do you have?
JonahAnimal
General
4
Which species have you done diet analysis for?
Can you find out more than just diet from a fecal sample?
Can you determine what lichens my animal is eating?
How do you look at what is in a honey sample?
Sampling
15
What is the best way to sample fecal material?
Do I need to know exactly how many samples I will be submitting ahead of time?
Can I send you samples in other containers such as plastic bags?
What if I am sending swabs and they don’t fit?
Should I write more information on the vial?
View all 15
Lab
5
What primer pairs do you use?
Should I run replicates?
Should I keep duplicates?
How many PCR replicates do you normally run?
Do you archive samples?
Analysis
0
JonahPlant
Lab
2
What primer pairs do you use?
Where do I ship samples?
JonahWater
General
4
How long does DNA stay in the water?
How far downstream from an organism can we detect the DNA?
What fish species do you have in your library?
There are over 1300 fish species in the United States. Your library only has about 500. What are you doing to fix the discrepancy?
Sampling
16
What do you include when you send a box of supplies for sampling?
What is the sampling protocol for eDNA?
Are there any aids in pushing water through the syringe? My hand is starting to hurt.
Can we use filters with different pore sizes?
How should samples be stored?
View all 16
Lab
5
What species can you detect with qPCR?
What primer pairs do you use for NGS?
Where and how do I ship my samples?
Do you archive samples?
What taxa can you detect with NGS?
Analysis
3
Can you calculate IBIs with eDNA data?
Why didn't you find all of the species at my site?
Why did you find something that isn't at my site?
Source Tracking
General
5
What assays do you have for Source-Tracking?
How do your methods compare to products that detect Enterococcus, E. coli, and fecal coliforms?
Is there a difference between what Jonah Ventures can find out and the fecal coliform tests?
Is this method certified by the EPA?
Are regionally specific libraries used to analyze fecal pollution sources?
Analysis
3
Can you separate the different strands of E. coli?
What are the taxa combinations Jonah Ventures can do?
What types of results can I expect?
Jonah Kits
General
8
Help, I can't log in to my account!
I haven't received an email about my account. Is something wrong?
Where can I access the data portal?
Will I receive a notification when my sample is received?
Can I get a replacement if I lost a kit component?
View all 8
JonahWater
6
What is in the eDNA sampling kits?
What is included in the educational materials for the JonahWater kit?
Do the kits have a shelf life?
At what temperature should the kits be kept at before use?
Are the kits susceptible to high/low temps?
View all 6
JonahAnimal
2
What is in your JonahAnimal kit?
What is included in the educational materials for the JonahAnimal kit?
NGS Reports
General
13
How do I read my NGS report?
What is an ESV?
Why are you switching from reporting OTUs to ESVs?
Are there any drawbacks to ESVs?
Should I consider every ESV to be real?
View all 13
Interpreting Data
1
How to interpret NGS data
qPCR Reports
General
1
How do I read my qPCR report file?
Consulting and Legal
Consulting
1
Can you help me with my eDNA project?
Legal
2
Would you be willing to sample as a neutral party?
Are your scientists non-biased?
Helpful Documents
qPCR
2
qPCR report explained
List of qPCR assays
NGS
2
NGS report explained
NGS Assays
Sampling Instructions
3
Water Sampling Instructions
Honey sampling instructions
Fecal sampling instructions
Classroom
3
Diet classroom activity and lesson plan
Aquatic eDNA lesson plan and activity (fish)
Educational fliers, fish and diet
Aquatic Documents
4
Macroinvertebrate traditional vs. eDNA methods
Aquatic eDNA method selection guide.
Source-tracking for waterkeepers flyer
NGS fish sequence library
Help Desk Software
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